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Living Modified Organism
(LMO)
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Flax modified for insertional mutagenesis
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Unknown
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Person:Agritec Plant ResearchZemedelska 2520/16Sumperk,
787 01, CzechiaPhone: +420 583 382-111,Fax: +420 583 382-999,Email: info@agritec.cz,Website: http://agritec.cz/new/index.php?lang=en,Related OrganizationAgritec Plant Research ()Private sector (business and industry)Zemedelska 2520/16Sumperk,
787 01, CzechiaPhone: +420 583 382-111,Fax: +420 583 382-999,Email: info@agritec.cz,Website: http://agritec.cz/new/index.php?lang=en,
Flax and linseed plants were genetically modified to induce mutations of existing plant genes using a vector for insertion mutagenesis. The vector contained a 35S tetramer enhancer fragment which, upon insertion, can cause an activation of the genes adjacent to the T-DNA insertion place, i.e. a dominant mutation. On the other hand the T-DNA integration into an active gene area may cause the inactivation of this gene. This change is then expressed as a recessive mutation. Theoretically these random mutations may result in changes in the plant's metabolism and in specific cases of it's morphological characteristics or the biological properties which may be useful for crop improvement.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12087-4 Organism Linum usitatissimum (Flax, Flax, Linseed, LINUS)Crops
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Binary vector GV3101 (pPM90RK)(pPCVRN4)
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-104314-4 Gene 4 transcription terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101504-4 CaMV 35S promoter plus four repeats of activating sequence | Cauliflower mosaic virus (CaMV)Promoter
The DNA segment inserted by the binary vector GV3101 (pPM90RK)(pPCVRN4) – does not contain any gene with direct effect on the recipient properties, other than a selectable marker, hpt, that confers resistance to hygromycin for the identification of transformed genotypes.
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- Research
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- Notification report: EU Joint Research Centre [ English ]
- Czech national node of the BCH [ English ]